Composite
Part:BBa_K209479:Design
Designed by: Katja Kolar Group: iGEM09_UCSF (2009-10-05)
pcDNA-SSFYFP-hM4D-Actinin1ABD
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 952
Illegal XbaI site found at 1065
Illegal SpeI site found at 249
Illegal SpeI site found at 935
Illegal PstI site found at 957
Illegal PstI site found at 2387
Illegal PstI site found at 5854
Illegal PstI site found at 6558
Illegal PstI site found at 7353 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 952
Illegal NheI site found at 895
Illegal NheI site found at 7589
Illegal SpeI site found at 249
Illegal SpeI site found at 935
Illegal PstI site found at 957
Illegal PstI site found at 2387
Illegal PstI site found at 5854
Illegal PstI site found at 6558
Illegal PstI site found at 7353
Illegal NotI site found at 978 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 952
Illegal BglII site found at 12
Illegal BglII site found at 5636
Illegal BglII site found at 8004
Illegal BamHI site found at 929
Illegal XhoI site found at 1059 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 952
Illegal XbaI site found at 1065
Illegal SpeI site found at 249
Illegal SpeI site found at 935
Illegal PstI site found at 957
Illegal PstI site found at 2387
Illegal PstI site found at 5854
Illegal PstI site found at 6558
Illegal PstI site found at 7353 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 952
Illegal XbaI site found at 1065
Illegal SpeI site found at 249
Illegal SpeI site found at 935
Illegal PstI site found at 957
Illegal PstI site found at 2387
Illegal PstI site found at 5854
Illegal PstI site found at 6558
Illegal PstI site found at 7353
Illegal NgoMIV site found at 1497
Illegal NgoMIV site found at 2838
Illegal NgoMIV site found at 3121
Illegal NgoMIV site found at 7460
Illegal AgeI site found at 1013 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 5592
Illegal BsaI.rc site found at 882
Illegal BsaI.rc site found at 4646
Illegal SapI site found at 3566
Illegal SapI.rc site found at 2687
Illegal SapI.rc site found at 2897
Design Notes
Cloned into BBa_K209473.